Determination of total alkaloid content in unpurified Dhattura beeja (Datura metel L. seeds) and purified Dhattura beeja (D. metel L. seeds) by UV-spectroscopic method

Poornachandra Tejaswini; Hassan Rangaswamy Pradeep; Divya Khare; Avinash Math

doi:10.4103/jdras.jdras_92_22

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ORIGINAL ARTICLE

Year : 2023 \| Volume : 8 \| Issue : 2 \| Page : 181-189

Determination of total alkaloid content in unpurified Dhattura beeja (Datura metel L. seeds) and purified Dhattura beeja (D. metel L. seeds) by UV-spectroscopic method Poornachandra Tejaswini¹, Hassan Rangaswamy Pradeep², Divya Khare³, Avinash Math⁴ ¹ ALN Rao Memorial Ayurvedic Medical College, Koppa, Chikmagalur District, Karnataka, India ² Department of Dravyaguna, ALN Rao Memorial Ayurvedic Medical College, Koppa, Chikmagalur District, Karnataka, India ³ Department of Dravyaguna, KAHER’s Shri B. M. Kankanawadi Ayurveda Mahavidyalaya, Belagavi, Karnataka, India ⁴ Department of Biochemistry, USM KLE International Medical Programme, Nehru Nagar, Belagavi, Karnataka, India Correspondence Address: Dr. Divya Khare Department of Dravyaguna, DTL, Central Research Facility, KAHER’s Shri B. M. Kankanawadi Ayurveda Mahavidyalaya, Belagavi, Karnataka India Source of Support: None, Conflict of Interest: None DOI: 10.4103/jdras.jdras_92_22

BACKGROUND: Dhattura herbal tea has been reported for toxicity, which is attributed to use without purification. Purification of Dhattura as per classics is essential as it contains toxic alkaloids. The present study highlights the role of classical Shodhana procedures in rendering the drug safe for therapeutics. METHODS: Dhattura seeds were collected and purified by Swedana (sudation) using Goksheera (cow milk) and Gomutra (cow urine). The unpurified and purified Dhattura seeds were analyzed for alkaloid content by UV spectroscopy. RESULTS: Unpurified Dhattura seeds contained 40.83 mg atropine equivalent (AE)/100 g of drug. Purification using Goksheera reduced alkaloid content to 20.46 mg AE/100 g of drug, and purification using Gomutra resulted in 26.45 mg AE/100 g of drug. CONCLUSION: UV spectroscopy is an effective method to determine the total alkaloid content in the raw and purified Dhattura seeds. This study supports that the purificatory methods stated in Ayurveda are effective in reducing atropine levels in Dhattura seeds.



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