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Year : 2023  |  Volume : 8  |  Issue : 2  |  Page : 173-180

Evaluation of the anti-inflammatory activity of hexane and ethanolic extracts of polyherbal formulation of Nigella sativa L. (seeds), Ocimum tenuiflorum L. (leaves), and Piper longum L. (fruits) on carrageenan-induced paw edema in wistar rats

1 Department of Pharmacy, Rajiv Gandhi Proudyogiki Vishwavidhyalay, Bhopal, India
2 Department of Zoology, Sri Satya Sai University of Technology and Medical Sciences, Sehore, Madhya Pradesh, India

Correspondence Address:
Abu Tahir
Department of Pharmacy, Rajiv Gandhi Proudyogiki Vishwavidhyalay, Bhopal 462033, Madhya Pradesh
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/jdras.jdras_85_22

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BACKGROUND: Inflammation is a primary physiological defence mechanism that assists the body in protecting itself from infection, toxic chemicals, or other noxious stimuli. The current study demonstrated phytochemical screening, anti-inflammatory activities, and sub-acute toxicity of hexane and ethanol extracts of Nigella sativa L. (seeds), Ocimum tenuiflorum L. (leaves), and Piper longum L. (Fruits), as well as anti-oxidant activity. The Carrageenan-Induced Rat Paw Edema method was used to assess anti-inflammatory activity. METHODS: The carrageenan-induced paw edema test evaluated anti-inflammatory activity. Male albino Wistar rats weighing 150 ± 10g were divided into six groups of six animals each. Paw edema was induced with 1.5% carrageenan in all the groups except the normal. Group, I received a plain control of 1 ml of 1% Carboxy Methyl Cellulose (CMC); Group II standard drug received Indomethacin (10 mg/kg); Group III received Polyherbal formulation of hexane extracts (PHFH) 250 mg/kg b.w; Group IV received PHFH 500 mg/kg b.w.; Group V was given 250 mg/kg b.w. of Polyherbal formulation of ethanolic extracts (PHFE). The extract’s acute toxicity (2000 mg/kg) as per OECD guidelines was studied in albino rats for 14 days. The qualitative analysis of various phytochemical constituents of various phytoconstituents was determined. The DPPH method was used to evaluate anti-oxidant activity. RESULTS: The results showed that both PHFH and PHFE exhibited marked inhibition of the edema size from 1, 3, and 5 hrs of study as compared to the standard drug indomethacin (10 mg/kg b.w). The PHFE (250 and 500 mg/kg) displayed excellent protection against inflammation to PHFH (250 and 500 mg/kg). Compared to the standard drug, indomethacin which showed the highest excellent protection against inflammation. PHFE has lower anti-oxidant activity than standard ascorbic acid but exhibits higher anti-oxidant activity than PHFH. In an acute toxicity test, hexane-ethanolic extracts up to 3000 mg/kg had no toxic effects. CONCLUSION: From this study, we conclude that Nigella sativa L. (seeds), Ocimum tenuiflorum L. (leaves), and Piper longum L. (fruits) have anti-inflammatory activity by reducing paw inflammation as well as showing anti-oxidant activity.

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