| ORIGINAL ARTICLE |
|
| Year : 2021 | Volume
: 6
| Issue : 4 | Page : 248-257 |
|
|
Identification and estimation of vicine bioactive compound in Momordica charantia L. fruit and coded Ayurvedic formulation using HPLC method
Ajay Kumar Meena1, Arjun Singh2, Kudingila Narasimha Swathi3, Vikas Ojha1, Amit Kumar Dixit4, Raju Ilavarasan3, Narayanam Srikanth2
1 Regional Ayurveda Research Institute, Gwalior, Madhya Pradesh, India
2 Central Council for Research in Ayurvedic Sciences, New Delhi, India
3 Captain Srinivasa Murthy Central Ayurveda Research Institute, Chennai, Tamil Nadu, India
4 Central Ayurveda Research Institute, Kolkata, West Bengal, India
Correspondence Address:
Ajay Kumar Meena
Regional Ayurveda Research Institute, Gwalior, Madhya Pradesh
India
 Source of Support: None, Conflict of Interest: None
DOI: 10.4103/jdras.jdras_19_21
|
|
|
BACKGROUND AND OBJECTIVE: Momordica charantia L. is one of the important Ayurvedic drugs having anti-diabetic, anti-viral, anti-ulcerogenic, anti-tumor, immunomodulatory, and anti-lipolytic and hepatoprotective properties. This study was designed for comparative fingerprint profiling of vicine bioactive compound in M. charantia L. fruit extract and coded Ayurvedic formulation extract through high-performance thin layer chromatography (HPTLC) and for developing rapid high-performance liquid chromatography (HPLC) method for the determination of vicine in fruit extract and coded Ayurvedic formulation. MATERIALS AND METHODS: The dried powder of M. charantia L. fruit (10.3448 g) and powder of coded Ayurvedic formulation (13.4975 g) were extracted by using a Soxhlet apparatus for 24 h. For HPTLC finger printing, ethyl acetate: methanol: water: formic acid (7:3:1:0.5; v/v/v/v) was used as a solvent system. The quantitative analysis was performed by HPLC using acetonitrile and water (65:35 v/v) as mobile phase, with 1.0 mL/min flow rate at 275 nm, and the retention time of vicine was 3.688 min. RESULTS AND DISCUSSION: A band was obtained at 254 nm (green, Rf = 0.31) in standard and test solution tracks of M. charantia L. fruit extract and coded Ayurvedic formulation extract corresponding to vicine. The calibration plot showing linear relationship with concentration has been observed. A linear equation is y = 12.224x + 7.829, and the goodness of fit (r2) of 0.99 was obtained from the regression analysis, which showed proportional dependence between concentration and peak area. CONCLUSION: Results of the HPLC analysis showed that the coded Ayurvedic formulation extract and M. charantia L. fruit extract contains 0.0273% and 0.0603% of vicine biomarker compound, respectively. |
|
|
|
| [FULL TEXT] [PDF]* |
|
 |
|
