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ORIGINAL ARTICLE
Year : 2021  |  Volume : 6  |  Issue : 4  |  Page : 248-257

Identification and estimation of vicine bioactive compound in Momordica charantia L. fruit and coded Ayurvedic formulation using HPLC method


1 Regional Ayurveda Research Institute, Gwalior, Madhya Pradesh, India
2 Central Council for Research in Ayurvedic Sciences, New Delhi, India
3 Captain Srinivasa Murthy Central Ayurveda Research Institute, Chennai, Tamil Nadu, India
4 Central Ayurveda Research Institute, Kolkata, West Bengal, India

Correspondence Address:
Ajay Kumar Meena
Regional Ayurveda Research Institute, Gwalior, Madhya Pradesh
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jdras.jdras_19_21

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BACKGROUND AND OBJECTIVE: Momordica charantia L. is one of the important Ayurvedic drugs having anti-diabetic, anti-viral, anti-ulcerogenic, anti-tumor, immunomodulatory, and anti-lipolytic and hepatoprotective properties. This study was designed for comparative fingerprint profiling of vicine bioactive compound in M. charantia L. fruit extract and coded Ayurvedic formulation extract through high-performance thin layer chromatography (HPTLC) and for developing rapid high-performance liquid chromatography (HPLC) method for the determination of vicine in fruit extract and coded Ayurvedic formulation. MATERIALS AND METHODS: The dried powder of M. charantia L. fruit (10.3448 g) and powder of coded Ayurvedic formulation (13.4975 g) were extracted by using a Soxhlet apparatus for 24 h. For HPTLC finger printing, ethyl acetate: methanol: water: formic acid (7:3:1:0.5; v/v/v/v) was used as a solvent system. The quantitative analysis was performed by HPLC using acetonitrile and water (65:35 v/v) as mobile phase, with 1.0 mL/min flow rate at 275 nm, and the retention time of vicine was 3.688 min. RESULTS AND DISCUSSION: A band was obtained at 254 nm (green, Rf = 0.31) in standard and test solution tracks of M. charantia L. fruit extract and coded Ayurvedic formulation extract corresponding to vicine. The calibration plot showing linear relationship with concentration has been observed. A linear equation is y = 12.224x + 7.829, and the goodness of fit (r2) of 0.99 was obtained from the regression analysis, which showed proportional dependence between concentration and peak area. CONCLUSION: Results of the HPLC analysis showed that the coded Ayurvedic formulation extract and M. charantia L. fruit extract contains 0.0273% and 0.0603% of vicine biomarker compound, respectively.


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